Background: Burn out syndrome consists in physical and mental exhaustion observed in professionals whose work involves continuous contact with other people in response to chronic stress. Healthcare providers in intensive care units are exposed to this risk. Our objective is to assess the prevalence and identify risk factors of burn out among the medical and paramedical staff in intensive care unit. Methods: A multicenter analytical cross-sectional study was conducted among practitioners in four Anesthesia and Intensive Care units in Moroccan University Hospitals. The Maslach Burn out Inventory and a general questionnaire on demographic and professional variables data were used. Results: High scores for emotional exhaustion, depersonalization, and low score for professional accomplishment were observed in, respectively, 48%, 21% and 43% of cases corresponding to a high score of burn out of 70%. In multivariate analysis, resident physicians and nurses were most at risk of burn out. Disorganization of work, fear of malpractice and the unsatisfactory salaries were associated to a relative risk of burn out respectively of 1.88, 2.09 and 1.93. Conclusion: This study documents evidences of burn out syndrome in healthcare providers working in intensive care units, preventive strategies are therefore required based on an improvement in the organization of care.