Abstract Assessment of individuals’ psychological well-being and mental health is an important aspect of health promotion. The purpose of this study was to examine the relationship between perception of psychological well-being and general health report among Jordanian university students. A total of 1,108 students from six universities in Jordan were surveyed regarding psychological well-being and general health. The results showed that students perceived their psychological well-being as moderate. Psychological well-being subscales were negatively correlated with reports of physical pain, chronic infections, and previous or current treatment of a psychiatric illness. Male and female university students were similar in their perceptions of psychological well-being; however, they differed in their general health report. Perception of psychological well-being is an important component of university students’ health. The importance of psychological well-being is highlighted, and implications for mental health nurses are presented.